Figure 1: Blood meal antibiotics affect mosquito microbiota.
From: Antibiotics in ingested human blood affect the mosquito microbiota and capacity to transmit malaria
(a) qPCR quantification of midgut bacterial 16S rDNA every 24 h during a three blood-feed (‘BF’) course. Blood was supplemented with penicillin/streptomycin (‘PS always’), water (control, ‘C’) or PS in the first blood meal and water thereafter (‘PS 1st’). (b) 16S rDNA 454-pyrosequencing analysis of mosquito gut microbiota before blood meal (sugar fed, ‘SF’), 24 and 72 h after PS-treated or untreated blood meal and 24 h after an untreated second blood meal. Main bacterial genera (>5% in at least one sample) and families are indicated. (c) Rarefaction curve of the estimated number of species using the Chao1 method. (d) qPCR microbiota analysis using generic or taxon-specific 16S rDNA primers. Data show average from three (a–c) to six (d) independent experiments, error bars show s.e.m., n=15–25 mosquitoes per sample and per replicate. qPCR data show fold versus day 0 (a) and % of control BF. (d) Enterobact.—Enterobacteriaceae; Flavobact.—Flavobacteriaceae; Acetobact.—Acetobacteraceae.
