Figure 3: Quality comparison of MARQUIS on the MRM versus PRM platform.

Observed versus expected total iTRAQ signal contribution for MRM and PRM methods for two sites: EGFR pY1173 (a) and Shc pY317 (b). Data represents mean and s.d. across five technical replicates. (c) CV versus amount for MRM and PRM methods. (d) Fraction of measurements that meet CV thresholds for both techniques.