Figure 3: Classification of subtomograms from wild-type mitochondria.
From: Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography
(a) In a first round, classification was focused on the 37S subunit of the mitoribosome (classification mask depicted in red) and separated 73S mitoribosomes from 54S subunits and mitoribosomes with a hyper-rotated 37S subunit. (b) In a second round, classification was focused on the membrane and parts of the 54S subunit (classification mask depicted in red) and enriched subtomograms with clear density for the inner mitochondrial membrane. Absence of the membrane density is mostly due to orientations of particles that do not allow resolving the membrane as a consequence of the ‘missing wedge effect’ in CET. Class abundance is given as percentage of all particles. For classification round 1, the membrane region of the subtomogram average is not shown.
