Figure 5: Resolution assessment via FSC and cross-resolution.
From: Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography
(a) The resolution of the subtomogram average was determined to 28 Å by FSC 0.5 criterion with a mask, encompassing the whole 73S mitoribosome and most of the inner mitochondrial membrane. For validation, the cross-resolution between the yeast 73S mitoribosome as determined by single particle cryo-EM9 and the 73S part of the subtomogram average was determined to 23 Å (FSC 0.33 criterion). (b) Local resolution assessment of the subtomogram average with an estimated range of 22 Å at the core of the mitoribosome and ~30 Å in the mitoribosome-associated membrane region.
