Figure 2: Sorting of infectious viruses by flow virometry.
From: Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles
(a,b) JUNV-A647 particles were sorted directly onto carbon-coated EM grids and labelled with the GPC-specific GD01 antibody followed by a secondary antibody and then revealed by Protein A 10 nm gold beads. The low (a) and high (b) fractions were negatively stained and analysed by transmission electron microscopy. Several particles per micrograph are shown and the images are representative of each population. The low fraction viruses are about 60 nm in diameter, while the high fraction viruses are around 150 nm. (c) TRPS was performed on unsorted (blue bars) or sorted particles smaller than 300 nm (pink bars). The histograms show the distribution of particle size detected in each sample.
