Figure 4: Correlation between GPC levels and RNA content.
From: Sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles
FVA of JUNV particles stained with Alexa 647 GPC antibody only (a) or YOYO-1 only (b). Dot plots represent nucleic acid dye fluorescence (x axis) as a function of the Alexa 647 GPC fluorescence (y axis). (c) Flow virometry analysis of particles stained with both Alexa 647 GPC antibody and the nucleic acid dye YOYO-1. (d) Pie charts representing the percentage of total RNA+ vesicles (green) and the percentage of potentially infectious particles (GPC+ RNA+; yellow) in the low and high fractions. The high fraction contains about 2.5 times more virions with both genetic material and surface GPC than the low fraction. (e,f) Relative amount of RNA contained in 20,000 sorted particles from total RNA+ or GPC+ RNA+ viral particles. Reverse transcriptase RT-qPCR with primers targeting the short (e) or the large (f) segment of JUNV is shown. Error bars are the mean±s.d. of triplicates.
