Figure 1: A NChIP-seq protocol to generate genome-wide chromatin maps from low cell numbers.
From: An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations
(a) Overview of our improved ULI-NChIP-seq protocol and comparison with previously published NChIP-seq and low-input ChIP-seq protocols. Grey: steps associated with sample loss; orange: steps optimized to minimize sample loss. Extrapolation15 of H3K9me3 (b), H3K27me3 (c) and H3K4me3 (d) library complexity based on low-input and standard ChIP-seq libraries sequenced with various depth. SE reads, single-end reads.
