Figure 2: Correlation between standard and ULI-NChIP-seq libraries built from 10 3 to 10 5 ESCs. | Nature Communications

Figure 2: Correlation between standard and ULI-NChIP-seq libraries built from 10 3 to 10 5 ESCs.

From: An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations

Figure 2

Genome browser screenshots of H3K9me3 (a), H3K27me3 (b) or H3K4me3 (c) profiles at the indicated genomic locations. (d) Genome-wide Pearson correlation (50,000 random 2 kb bins) between H3K9me3, H3 and H3K27me3 enrichment (RPKM) in data sets generated from 103 to 106 cells as input material. *Library prepared using the ‘gold-standard’ NChIP-seq protocol. ** and ***Technical replicates. †Obtained from ref. 35. (e) Pearson correlation of H3K4me3 enrichment (RPKM) around genic promoters (RefSeq TSS ±500 bp) between ULI-NChIP-seq libraries generated from 5 × 103 to 5 × 105 cells and ENCODE libraries18 in E14 mouse ESCs.

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