Figure 3: High-resolution gender-specific H3K27me3 profiles generated from E13.5 PGCs isolated from single embryos.
From: An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations
(a) Library complexity of H3K27me3 data sets prepared from E13.5 PGCs using ULI-NChIP-seq compared with previously published data sets generated using alternative low-input XChIP-seq protocols (*ref. 13; **ref. 13; ***ref. 12). The number of input cells is indicated above each bar. (Rep.: Repeat) (b) Genome browser screenshots of H3K27me3 enrichment around the HoxC cluster illustrating the complexity of libraries generated using ULI-NChIP-seq library and correlation to previously published data sets12,13 generated using an alternative low-input ChIP-seq protocol (*ref. 13; **ref. 13; ***ref. 12). (c) Pearson correlations between H3K27me3 data generated from 103 male PGCs using our low-input protocol and previously published data around gene promoters (RefSeq TSS ±2 kb). (*ref. 13; **ref. 13; ***ref. 12).
