Figure 4: Gender-specific H3K27me3 profiles from E13.5 PGCs isolated from single embryos.
From: An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations
Relationship between gene promoter enrichment of H3K27me3 (RPKM, RefSeq TSS ±1 kb) and gene expression (exonic RPKM) in male (a) and female (b) E13.5 PGCs. Enrichment of H3K27me3 in genic promoter regions (RPKM, RefSeq TSS ±1 kb) (c) and expression (exonic RPKM) of annotated genes (d) in male versus female PGCs. H3K27me3-marked genes that show an inverse relationship with expression in male (gold) and female (red) PGCs are highlighted. (e) Genome browser screenshots of male versus female H3K27me3 enrichment and gene expression at selected loci, revealing sex-specific H3K27me3-associated gene silencing in E13.5 PGCs.
