Figure 3: Jas9-VENUS can be used to study long-distance JA signalling in planta.

(a) Seedlings were wounded by cutting a cotyledon using dissection scissors. Time-course quantification of Jas9-VENUS fluorescence, normalized to H2B-RFP fluorescence, in the inner and outer tissues of the root over 120 min after wounding. Error bars show the s.e.m. of 13 replicates. (b) Time-course quantification of Jas9-VENUS fluorescence, normalized to H2B-RFP fluorescence, in the inner tissues at four positions along the root (indicated on the diagram) before and after wounding. (c) Shoots and roots of 1-week-old WT or aos seedlings were harvested 30 min and 2 h after wounding and the expression of wound-inducible genes were investigated using RT–qPCR. The experiment was repeated twice and the results show a representative data set. Error bars represent the s.d. of four technical replicates.