Figure 1: YAP abundance decreases with postnatal maturation of skeletal muscle fibres.
From: The Hippo pathway effector YAP is a critical regulator of skeletal muscle fibre size
(a) Representative western blots showing YAPS112, YAP and GAPDH levels from embryonic day 17 (E17) throughout postnatal maturation until 42 days of age. n=3. (b) Yap mRNA expression between E17 and 4 weeks of age (mean±s.e.m., n=5, P=0.0007; unpaired t-test). (c) Representative image of YAP localization in the TA muscle of an 8-week-old C57BL/6 mouse, (d) Laminin labelling marking the muscle fibre membrane, (e) 4′,6-diamidino-2-phenylindole (DAPI)-labelled nuclei, (f) Merged image of c–e. YAP is shown in red, DAPI in blue and laminin in grey. Arrows indicate YAP-positive muscle nuclei, (g) negative control of tissue section labelled with secondary antibody and DAPI only. (h–l) higher magnification images of YAP localization in muscle nuclei and diffuse membrane localization, accumulating at the membrane near the acetylcholine receptors (AchR) as labelled with α-bungarotoxin conjugated to AlexaFluor594 and shown in green in l. Scale bar (c,g), 100 μm and (h–l), 25 μm. n=3.
