Figure 3: Ezh2 is not required for normal melanocyte homeostasis. | Nature Communications

Figure 3: Ezh2 is not required for normal melanocyte homeostasis.

From: The epigenetic modifier EZH2 controls melanoma growth and metastasis through silencing of distinct tumour suppressors

Figure 3

(a,b) Mouse genotypes (a) and strategy (b) used to analyze the effect of conditional Ezh2 ablation in the melanocytic lineage of adult wild type mice. (c) Macroscopic pictures and H&E staining on trunk skin sections of control and cKO mice at 5 months and 1 year post conditional Ezh2 ablation. (d) Immunofluorescent staining on trunk skin sections of wt mice for β-Gal to quantify recombination efficiencies (representative fields not shown). (e) Immunofluorescent staining on trunk skin sections of wt mice for Sox10 (control) or β-Gal (cKO) and H3K27me3 to quantify H3K27me3 depletion. White arrowhead, Sox10-positive cell considered H3K27me3 positive; white open arrowhead, β-Gal-positive cell considered H3K27me3 negative. H&E, haematoxylin and eosin; HF, hair follicle. Data are represented as mean±s.e.m. of n=3. P values calculated with unpaired Student’s t-test. Scale bars, 50 μm.

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