Figure 9: The EZH2 target gene AMD1 is a tumour suppressor that prevents melanoma metastasis. | Nature Communications

Figure 9: The EZH2 target gene AMD1 is a tumour suppressor that prevents melanoma metastasis.

From: The epigenetic modifier EZH2 controls melanoma growth and metastasis through silencing of distinct tumour suppressors

Figure 9

(a) Kaplan–Meier curves comparing overall survival of melanoma specimens (stage I–IV) with respect to AMD1 transcript levels based on TCGA. (b) Kaplan–Meier curves comparing distant metastases-free survival of stage I–III melanoma specimens (primary melanoma/lymph node metastases) with respect to AMD1 transcript levels based on TCGA. (c) Breslow depths of melanoma specimens’ primary melanomas with respect to AMD1 transcript levels based on TCGA. (d) Reverse transcription–qPCR (RT–qPCR) for EMT genes on M990514 after AMD1 depletion with siAMD1. (e) RT–qPCR for EMT genes on B16-F1 after Amd1 depletion with shAmd1. (f) Mouse genotypes and strategies used to i.v. engraft B16-F1/B16-F10 cells following Amd1 silencing using shAmd1 to analyze the effect of Amd1 depletion and GSK503 treatment on metastases formation. (g) Representative macroscopic pictures of lungs from C57Bl/6 animals after i.v. engraftment of shCo and shAmd1 B16-F1 cells to quantify lung metastases. (h) Representative macroscopic pictures of lungs from vehicle and GSK503-treated (from day 1 on until endpoint) animals to quantify lung metastases after i.v. engraftment of shCo and shAmd1 B16-F10 cells. AMD1 low/high, bottom and top 60 patients with respect to AMD1 transcript levels; i.v., intravenous; shA, shAmd1. Data are represented as median±100% range of n=48 (AMD1 high), n=43 (AMD1 low) (c), mean±s.e.m. of n=3 (d,e), mean±s.e.m. of n=4 (g,h), n=3 (shCo, GSK503 in h). P values calculated with log-rank (Mantel–Cox) test (a,b), unpaired Student’s t-test (c), analysis of variance and Fisher’s least significant difference test (d,e,g,h).

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