Figure 9: Inhibition of glycolysis decreased viability and ATP steady-state levels in lines expressing high MnSOD levels. | Nature Communications

Figure 9: Inhibition of glycolysis decreased viability and ATP steady-state levels in lines expressing high MnSOD levels.

From: MnSOD upregulation sustains the Warburg effect via mitochondrial ROS and AMPK-dependent signalling in cancer

Figure 9

Cells were treated with the glucose competitor 2-DG (10 mM for 36 h) and analysed by flow cytometry using YOPRO, an indicator of apoptosis and propidium iodide, an indicator of cell death. (a) Cells overexpressing MnSOD were more susceptible to 2-DG-induced apoptosis compared with control cells (neo). Mn44 and Mn11 cells as expected were the most vulnerable to glycolysis inhibition. (b) In cells treated with 2-DG, there were also decreases in steady-state ATP levels analysed by chemiluminescence 36 h after 2-DG addition to the media. Reductions in ATP levels were marked in Mn44 and Mn11 confirming the reliance of these cells on glycolysis for ATP production. Statistical analysis was performed using one-way ANOVA (GraphPad InStat) **P<0.01; N=3 independent experiments.

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