Figure 2: IRAK-M suppresses the NTHi-induced proinflammatory mediator expression.
From: Glucocorticoids suppress inflammation via the upregulation of negative regulator IRAK-M
(a) The proteins from BEAS-2B cells stably overexpressing IRAK-M and its control pcDNA3.1 (Mock) cells were detected by immunoblot with indicated antibodies. (b) The mRNA expression was detected by qPCR after the stable cells were treated with DEX (100 nM) and NTHi. (c) BEAS-2B cells were transfected with siRNA control or IRAK-M for 72 h. IRAK-M protein expression was detected by immunoblot with anti-IRAK-M antibodies (SC, Santa Cruz Biotechnology sc-100389; PS, ProSci #2355). (d) siRNA-transfected BEAS-2B cells were infected with NTHi followed by qPCR. (e) The mRNA level was calculated by following: (NTHi-induced mRNA at each concentration of DEX)/(NTHi-induced mRNA without DEX). Data in b,d,e, n=3) are mean±s.d. *P<0.05; t-test.
