Figure 4: Functional analysis of the ORCO N-terminus. | Nature Communications

Figure 4: Functional analysis of the ORCO N-terminus.

From: Amino acid coevolution reveals three-dimensional structure and functional domains of insect odorant receptors

Figure 4

(a–c) Left: representative whole-cell current traces to the indicated stimuli with two-electrode voltage-clamp in Xenopus oocytes injected with cRNAs for the indicated combinations of wild-type or mutant D. melanogaster ORs. ORCO6Mut contains amino acid substitutions in the six top-ranked N-terminal residues (A23S, M24A, F30A, M31A, H32A and N33A); ORCOΔ23–33 bears a deletion of this region (A23–N33). Ligand solutions were applied for 3 s (arrowheads). Right: quantification of current amplitudes (mean±s.e.m.; n=5 oocytes for wild-type ORCO and ORCO6Mut; n=4 for ORCOΔ23–33).

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