Figure 4: mTOR–eIF4F key players are localized at the CTA.
From: Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR–eIF4F pathway
(a) mTOR (green) localizes with HPG signal (red) at the CTA. (b) Immunocytochemistry shows the localization of mTOR–eIF4F pathway components 2 h post NEBD. White line indicates oocyte cortex; representative images of at least three independent experiments are shown; scale bars, 20 μm. See also Supplementary Fig. 6. (c) ER tracker shows perispindular localization of ER and overlaps with the PTA post NEBD. Polymerized LMN separates CTA from PTA. HPG, LMN, MB (red); 4E-BP1(T70), eIF4E(S209) green; ER tracker and DAPI (blue). Data are representatives of at least two independent experiments; white line indicates oocyte cortex; scale bar, ~10 μm.
