Figure 6: Downregulation of mTOR and 4F abolishes the translation at the CTA.

(a,b) Inhibition of mTOR or 4F decreases HPG fluorescence at the CTA, followed by quantification of HPG fluorescence (bold circle indicates measured area (CTA); thin circle (PTA)). Data are represented as mean±s.d.; **P<0.01 and ***P<0.001, according to a Student’s t-test; n≥20. Scale bar, 35 μm. The overall translation was supressed by puromycin. (c) Measurement of phosphorylation intensity of the 4E-BP1 (T70) shows a decrease at the CTA in the presence of Rap (***P<0.001; n≥19). 4EGI does not affect the phosphorylation intensity significantly (P>0.1; n=20). Bold circle indicates measurement at the CTA and thin circle at PTA/cytoplasm. Data represents the mean ±s.d.; asterisks denote statistically significant differences Student’s t-test. (d,e) Downregulation of the 4F pathway results in decreased translation of selected mRNAs. Data are representative of at least three independent experiments. Values obtained for GV stage were set as 100%. Data are represented as the mean±s.d. **P<0.01, according to a Student’s t-test. (f,g) Immunoblot analysis of the candidate proteins during meiotic maturation. Values obtained for GV stage were set as 100%, n≥3. Data represents the mean ±s.d.; asterisk denotes statistically significant differences Student’s t-test: P<0.05. See also Supplementary Fig. 10.