Figure 7: The oocyte nucleus stores a pool of RNA.
From: Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR–eIF4F pathway
(a) RNA FISH shows the presence of a poly(A)-RNA population in the nucleus and in the vicinity of chromosomes in GV and NEBD stage oocytes. Poly(A) (red); DAPI (Blue). See also Supplementary Fig. 8. Scale bar, 20 μm. (b) MB shows the presence of poly(A)-RNA in the nucleus and in the vicinity of chromosomes in the GV oocyte and 2 h after NEBD. MB (red); H2B-GFP (green fluorescent protein; green). Scale bar, 20 μm. See also Supplementary Movie 2. (c) The oocyte nucleus contains specific mRNAs. RNA isolated from the nuclear (N) and cytosolic (C) fractions shows the presence or absence of specific transcripts by PCR. Data represents at least three independent experiments. Representative images of the isolated nuclei from mouse oocytes are shown. Scale bar, 10 μm. See also Supplementary Fig. 9 and Supplementary Table 1. (d) RNA FISH shows nuclear absence of Camk2a, cMos, Gapdh and nuclear presence of Bub3, Nph1, Survivin, Dazl mRNAs. The white line indicates the border of oocyte cortex; the white dashed line indicates the border of the nucleus; representative images of two independent experiments shown. Bar size, 20 μm.
