Figure 2: DN-MCU gene painting impairs ISO rate response in isolated hearts.
From: The mitochondrial uniporter controls fight or flight heart rate increases
(a–b) Immunofluorescent staining of (a) control right atrium and (b) gene-painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bar, 200 μm. (c,d) Example ex vivo ECG tracings recorded under basal (left) and ISO-stimulated (right) conditions from gene-painted hearts. Scale bar, 100 ms. (e) Summary dose–response data for ex vivo heart rate response to ISO. (f) Percentage rate increases over baseline after ISO in gene-painted isolated, Langendorff-perfused hearts (data calculated from e). ***P<0.001, unpaired Student‘s t-test, n=22–24 per group in e. Error bars indicate s.e.m.
