Figure 4: Confocal line-scan measurements of spontaneous calcium transients and caffeine-induced calcium release in SAN cells isolated from WT and DN-MCU mice.
From: The mitochondrial uniporter controls fight or flight heart rate increases
(a) Typical calcium transient images and spatially averaged traces in spontaneous beating SAN cells. (b) Typical caffeine-induced calcium release images and spatially averaged traces. (c) Summaries of spontaneous diastolic calcium release events and SR calcium content before and after ISO. Scatter graph of diastolic calcium events before and after ISO (upper panel). Bar graph of diastolic calcium release events before and after ISO (middle panel). Bar graph of SR calcium content determined by caffeine-evoked calcium release before and after ISO (lower panel). *P<0.05, **P<0.01 versus control, #P<0.05 versus WT by Student’s t-test, n=11–21 cells per group.
