Figure 7: ISO increases SAN cell mitochondrial Ca2+.
From: The mitochondrial uniporter controls fight or flight heart rate increases
(a) Adenovirus infected SAN cells expressing mt-pericam co-localized with MitoTracker (scale bar, 10 μm). (b) The mt-pericam fluorescence signal was increased by ISO (100 nM) stimulation and Ru360 dialysis prevented this increase (scale bar, 10 μm). (c) Example tracings show ISO increases in mitochondrial [Ca2+] ([Ca2+]mito) were prevented by Ru360 dialysis (scale bar, 100 s). (d) Summary data for [Ca2+]mito responses to ISO. ***P<0.001, n=14–15 per group, unpaired Student‘s t-test. Error bars indicate s.e.m.
