Figure 8: Ru360 prevents ISO-stimulated increases in NADH.
From: The mitochondrial uniporter controls fight or flight heart rate increases
(a) Example of NADH fluorescence responses to ISO (100 nM) in control (–Ru360) and Ru360 dialysed SAN cells. Scale bar, 100 s. (b) Summary data for NADH fluorescence responses to ISO. ***P<0.001, n=7–18 per group, unpaired Student‘s t-test. Error bars indicate s.e.m. (c) Proposed mechanism for MCU-enhancing SAN responses to ISO. β-AR, beta adrenergic receptor; CaV, voltage-dependent Ca2+ channels; DN-MCU, dominant-negative MCU; HCN, hyperpolarization-activated cyclic nucleotide-gated channels; MCU, mitochondrial calcium uniporter; N27A, super-inhibitory phospholamban; NCX, Na+/Ca2+ exchanger; PLN, phospholamban; SERCA, sarco/endoplasmic reticulum Ca2+-ATPase; SR, sarcoplasmic reticulum; TG, thapsigargin.
