Figure 5: GTR1-mediated transport of hormones into Xenopus oocytes.

(a) GTR1-mediated transport of hormones. Twenty-four hours after cRNA injection, oocytes were added to kulori-based buffer (pH 5.0) containing 100 μM JA, 100 μM TA, 100 μM JA-Ile, 100 μM ABA and 100 μM GA₃ and incubated at 17 °C for 24 h. Oocytes were washed with sorbitol solution, homogenized in extract medium and incubated at 4 °C for 24 h. After centrifugation, supernatants were collected. Samples were subjected to UPLC/TOFMS analysis. Control means water injection into Xenopus oocytes. Values are the mean±s.d. of eight (control) or nine (GTR1) biological replicates. *P<0.05; Student’s t-test. (b) Activities of GTR1 in the presence of 100 μM 4MTB. Values are the mean±s.d. of 17 biological replicates. *P<0.05, NS, not significant (P>0.05); Student’s t-test.