Table 3 Primers used in this study.
From: Origins of multicellular evolvability in snowflake yeast
Primer name | Strain (derivative of Y55) | Sequence (5′–3′) | Source |
|---|---|---|---|
ace2mx_F | ace2::NATMX4 | CAAAGAAATCTATAGGACCAAAAACGGTGTTAATACAATCCGTACGCTGCAGGTCGAC | This study |
ace2mx_R* | ace2::NATMX4 | ATTATTTACTATGTTAATATCATGCATAGATAAATGTTCGATCGATGAATTCGAGCTCG | This study |
ACE2wtmx_fusn_F | ACE2-KANMX | ACCAAAGGATGTGTGAAGCTGGTTTGTAGTAGTTAAAGGGATCGATGAATTCGAGCTCG | This study |
ACEwtmx_fusn_R* | ACE2-KANMX | ATTTCTTTACGATTTACGTACACTGTAGTCTTAAGGGCCACGTACGCTGCAGGTCGAC | This study |
ACE2wtmx_whole_F | ace2/ace2::ACE2-KANMX | CGTTGCAGGGAGACTCAA | This study |
ACE2wtmx_whole_R* | ace2/ace2::ACE2-KANMX | TTAGGGTTATGTCCCTATAAACGATGACTATTGCCTTTTTATCGATGAATTCGAGCTCG | This study |
