Table 2 In-process sample analysis (HIC-HPLC) of in-process purification samples for H2-1H2-2 at 100 l scale.

From: Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG

Purification step

% κκ mono

% λλ mono

% κλ body

% IgG (step/overall)

% κλ-body (step/overall)

MabSelect SuRe

42

13

45

93/93

93/93

LambdaFabSelect

0

11

89

47/43

89/83

KappaSelect

0

0

100

81/35

90/74

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