Figure 2: Oligodendrocyte development is impaired in gpr56 zebrafish mutants.
From: The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA
(a,b) Representative fluorescent images of (a) control (gpr56stl13/+) and (b) gpr56stl13/13 larvae at 28 hpf expressing Tg(sox10(−7.2):memGFP) to mark OPCs. (c) Quantification of OPC number (sox10+; white arrows) in control (N=20) and gpr56stl13/stl13 (N=19) larvae. (d–f) Representative WISH images of the spinal cord of (e) gpr56stl13/stl13 (N=19) and (d) control embryos (gpr56+/+ and gpr56stl13/+, N=22) showing nkx2.2a expression to mark pro-OLs. (f) Quantification of pro-OL number (nkx2.2a+) (P<5.223 × 10−6). (g–j) mbp expression (CNS marked by white arrow, hindbrain) in (h) gpr56stl13/13 (N=20/27), (i) gpr56stl14/stl14 (N=14/19) and (j) MZgpr56stl/13/stl13(5/5) larvae compared with (g) WT controls at 4 dpf (N=13/13). (a–f) All images and quantification thereof were taken from segments 5 to 6 for consistency. For (a,b,d,e) lateral views are shown, anterior to the left, dorsal is up; for (g–j) dorsal views are shown, anterior to the left. (a,b) Scale bar, 50 μm. (d,e) Scale bar, 100 μm. (g–j) Scale bar, 200 μm. Student’s t-test used to test for statistical significance and error bars shown as±s.d. A minimum of two technical replicates were performed for each marker. NS, not significant.
