Figure 5: Ser177 phosphorylation of Pdlim5 causes reorganization of lamellipodia, stress fibres and focal adhesions.
From: Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5
(a) GFP images (left) and immunostained images of KDR/EGFP-Pdlim5 cells (WT, S177A and S177D). Cells were stained with phalloidin and anti-paxillin antibody, to visualize actin microfilaments and focal adhesions, respectively. Magnified images outlined by yellow squares show the area outlined by the white squares. Scale bar, 10 μm. (b,c) Sequential GFP images of KDR/EGFP-WT-Pdlim5 (b) or KDR/EGFP-S177A-Pdlim5 (c) cells before and after AICAR stimulation (2 mM). Yellow arrowheads represent dorsal stress fibres growing from the opposite side after AICAR treatment of KDR/EGFP-WT-Pdlim5 cells. Scale bar, 10 μm.
