Figure 1: Neutralizing antibody targets on Env trimers.
From: Antibody potency relates to the ability to recognize the closed, pre-fusion form of HIV Env
(a) The structural elements of gp120 are coloured on one protomer of the closed, pre-fusion Env trimer (PDB accession code: 4NCO)10. (b) The epitopes of the various NAbs used in this study are highlighted on the structure of the closed, pre-fusion trimer. The CD4bs, the target of antibodies b12, VRC01 and PGV04 is shown in orange8,21,22. Glycans at N301 and N332 along the base of V3 make up the PGT123 epitope (magenta)41. The glycan at N160 and nearby residues in V1/V2 constitute the PG9 epitope (cyan)31. Antibody 2G12 recognizes the cluster of glycans at positions N295, N332, N392, N386 and N448 (green)28. Antibody 17b sees the CD4i epitope that is only formed and exposed after CD4 binding23. The viral membrane is shown at the base to illustrate the orientation of Env (electron microscopy databank (EMD) accession code: 5022)9. (c) The breadth (by per cent of viruses neutralized at concentrations <50 μg ml−1) and neutralization potency (median IC50 values of the viruses neutralized) are plotted for each NAb. Neutralization data were obtained from BNAber.org39.
