Figure 4: Kinetics of 17b Fab binding to SOSIP.664 trimers.
From: Antibody potency relates to the ability to recognize the closed, pre-fusion form of HIV Env
(a) BG505 and (b) KNH1144 SOSIP.664 trimers were incubated with ligands for various times (from 3 min to 72 h) and resolved by BN–PAGE. (c) 17b was incubated with KNH1144 SOSIP.664 trimers for various times, the complexes captured by protein G beads, resolved by SDS–PAGE and visualized by immunoblotting. Identical experiments performed in the presence of BMS-806, NBD-556 and sCD4 reveal that NBD-556 and sCD4 both accelerate 17b binding, while BMS-806 blocks it altogether. The gp120 band derived from SOSIP.664 trimers is in the highlighted region marked by the arrow. The other bands represent nonspecific binding of the anti-human serum used as the secondary antibody.
