Figure 2: Amplicons identified from genome sequencing coverages. | Nature Communications

Figure 2: Amplicons identified from genome sequencing coverages.

From: Macrotene chromosomes provide insights to a new mechanism of high-order gene amplification in eukaryotes

Figure 2

(a) Copy number variation along a chromosome VIII segment, as determined from sequence coverages of parental and evolved strains of the BYAT580 evolutionary experiment (see Methods). For strain numbering, refer to Table 1. BYAT580-265 and -345 are subclones of BYAT580-120 and -200, respectively, after 145 additional generations (see the text and Supplementary Fig. 10). Copy numbers (ordinates) were normalized to 2 (for diploids). Horizontal grey bars on top materialize amplicon units. Abrupt curve drops correspond to the Y. lipolytica Asn-RS gene (blue diamond) absent from the S. cerevisiae reference sequence. Pink oval: centromere. (b) Copy number variation along a chromosome IV segment, as determined from sequence coverages of parental and evolved strains of the BYAT583 evolutionary experiment. Same legend as in a, BYAT583-345 is a mutant subclone of BYAT583-201 (Table 1) after 145 additional generations (see the text and Supplementary Fig. 10). Blue diamond: Y. lipolytica Lys-RS gene. *Location of ENA tandem gene array (artificially cut for clarity). Note the complex pattern of amplifications along chromosome IV because of the superposition of several events.

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