Figure 3: Pulsed-field gel electrophoresis of parental and evolved strains. | Nature Communications

Figure 3: Pulsed-field gel electrophoresis of parental and evolved strains.

From: Macrotene chromosomes provide insights to a new mechanism of high-order gene amplification in eukaryotes

Figure 3

PFGE were run according to Methods with pulse ramps as indicated (linear accelerations). Left: ethidium bromide fluorescence. Size scale in Mb, calibrated from migration of natural S. cerevisiae chromosomes. Right: hybridization with indicated probes. (a) BYAT580 evolutionary experiment. Top diagrams: location of probes with respect to amplicons (box) and scheme of amplified structures observed: [C] normal chromosome (scheme in box), [M] macrotene chromosome (arbitrary repeat number), [E] circular episome, [*] hybridizing material in slot (including large circular episomes), [E#] broken episome (linearized DNA). Strains 1: BYAT580-0, 2: BYAT580-60, 3: BYAT580-120, 4: BYAT580-200. Note the variable migration of the circular episome relative to chromosomes with different pulse frequencies63. (b) BYAT583 evolutionary experiment. Same legend as explained in a, [SD] segmental duplication. Strains 1: BYAT583-0, 2: BYAT583-60, 3: BYAT583-120, 4: BYAT583-130, 5: BYAT583-170, 6: BYAT583-190, 7: BYAT583-200.

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