Figure 4: Junction sequences and repeat organizations in macrotene chromosomes.

(a) Macrotene chromosome VIII. Grey cylinders with a pink tightning (centromere) schematize normal (top) and macrotene (bottom) chromosome VIII. Amplicon VIII-B is symbolized by the yellow (left arm) and purple (right arm) boxes with internal arrows to indicate orientation. The centre part of figure details genetic elements present in the amplicon region (top) with zooms on junction regions (middle). Junction sequences are from Supplementary Fig. 6. Grey arrowhead boxes: protein-coding genes (Y. lipolytica Asn-RS genes is highlighted in red and labelled with N); pink oval: centromere; thick vertical black lines: tRNA genes; yellow, dark blue and orange boxes: delta, sigma and tau elements, respectively (orientation given by internal arrows). Filled or void light blue boxes locate replication origins (ARS), respectively, confirmed or proposed⁶⁴. (b) Macrotene chromosome IV. Grey cylinders with a pink tightning (centromere) schematize normal (top) and macrotene (bottom) chromosome IV. Amplicon IV-B is symbolized by purple boxes of vanishing intensity with internal arrows to indicate orientation. Centre part of figure, same legend as above. The Y. lipolytica Lys-RS gene is labelled K. Sequence of the right junction is indicated (black letters: palindromic part, red letters: unique central part, line indicates direct sequence continuity). Chr., chromosome.