Figure 3: BCR confinement and diffusion are controlled by the actin cytoskeleton. | Nature Communications

Figure 3: BCR confinement and diffusion are controlled by the actin cytoskeleton.

From: Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor

Figure 3

Ex vivo B cells, as well as B cells that had been cultured overnight with either 5 ng ml−1 BAFF or BAFF+5 μg ml−1 LPS, were treated with dimethylsulphoxide (DMSO) or 1 μM latrunculin B (LatB) for 3 min before performing SPT as in Fig. 2. (a) BCR trajectories were generated from 10-s 33 Hz videos (compare Supplementary Movies 4 and 5 to Supplementary Movies 1 and 2). Scale bar, 5 μm. Red, confined trajectories; cyan, free trajectories. (b,c) The per cent of mIgM BCRs that exhibited free diffusion is shown (b), along with the median diffusion coefficients for both confined and free mIgM-containing BCRs (c). Horizontal lines represent the mean values for >15 videos per condition; >300 trajectories per condition were analysed. ***P<0.001, **P<0.01, or NS (not significant) using Student’s 2-tailed unpaired t-test.

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