Figure 1: Carbohydrate-binding specificity of GAL1 free and bound to λ5-UR.

Fluorescently labelled GAL1 was analysed for binding to the mammalian glycan array (V5.1) of the CFG when free (a) or bound (b) to λ5-UR. Results shown for each plot are averages of two biological replicate measurements of fluorescence intensity at a lectin concentration of 5 μg ml⁻¹. Error bars indicate the s.e.m. for two experiments. The raw data and the entire list of glycans with the respective spacers can be found on the CFG website ( http://functionalglycomics.org/). Glycans presenting the highest relative fluorescence intensity are indicated with their number ID (The structure of these glycans is depicted in Supplementary Fig. 1). (c) Relative fluorescence intensity comparison for each glycan between free and λ5-UR bound GAL1. The fluorescence intensities measured in a and b have been normalized and the differential plot between free and bound GAL1 has been generated. Positive and negative bars indicate a higher and a weaker binding of GAL1, respectively, for the glycan in the presence of λ5-UR. Glycan structures and number ID are depicted for several epitopes with significant relative fluorescence difference and sharing similar structural motifs (clusters of bars presenting significant differences are numbered from (i) to (v)).