Figure 4: Mapping LNnT binding on GAL1. | Nature Communications

Figure 4: Mapping LNnT binding on GAL1.

From: Pre-B cell receptor binding to galectin-1 modifies galectin-1/carbohydrate affinity to modulate specific galectin-1/glycan lattice interactions

Figure 4

(a) Overlay of 2D 1H,15N HSQC spectra of GAL1 in the free form (blue) and with a fourfold molar excess of LNnT (red). Expanded regions show resonances representative of those that showed large CSPs on binding to LNnT. (b) CSPs induced by LNnT binding mapped onto the GAL1 surface (PDB 1GZW). Monomer surface structure is represented. CSPs have been coloured from blue to iodine (CSP<0.05: blue, <0.1 marine, <0.125: greencyan, <0.15: green, <0.175: lemon, <0.2: yellow orange, <0.225: orange, <0.25: red, <0.275: iodine). White is used for unassigned residue resonances. Subsites C–E of the GAL1 CBS are delineated with pink and yellow dotted lines. The λ5-UR22-45-interacting site is indicated using dotted grey lines.

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