Figure 1: PAXX is one component of the NHEJ complex. | Nature Communications

Figure 1: PAXX is one component of the NHEJ complex.

From: Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway

Figure 1

(a) A silver-stained SDS gel showing the polypeptides that were immunopurified with Flag antibody from the extracts of HEK293 cells expressing Flag-tagged XRCC4, XLF, PAXX, Lig4, Ku70 or Ku80. As a control, a mock IP (Mock) was performed using regular HEK293 cells that did not express Flag-tagged protein. The proteins identified using mass spectrometry and the number of peptides discovered for each protein are listed in Table 1. The Flag-tagged bait proteins in the gel are indicated by red arrowheads. (b) Immunoblotting shows that PAXX co-immunoprecipitates with Flag-Ku80. (c) Immunoblotting shows Flag-PAXX IP under different wash conditions with the indicated sodium concentrations. The numbers in b and c indicate the related intensity of the bands above. (d) The accumulation of green fluorescent protein (GFP)-PAXX at the laser microirradiation-induced damage sites in U2OS cells. RFP-Ku80 was included as a control. Images were obtained before (left panel) and 3 min after (right panel) irradiation. Scale bars, 2 μm. (e) Immunoblotting of the IP with endogenous antibodies.

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