Figure 3: PAXX interacts with the Ku complex and the XLF–XRCC4–Lig4 complex through its CTR. | Nature Communications

Figure 3: PAXX interacts with the Ku complex and the XLF–XRCC4–Lig4 complex through its CTR.

From: Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway

Figure 3

(a) Schematic representation of different PAXX mutants (left) and their ability to coimmunoprecipitate with Ku and XLF–XRCC4–Lig4 complexes from HEK293 extracts (right). Mutation sites are indicated in Supplementary Fig. 1a. (b) An IP–WB analysis to determine whether PAXX mutants in a coimmunoprecipitate with different NHEJ proteins. (c) Imaging shows that the PAXX mutant F201A loses its localization at laser microirradiation-induced damage sites. RFP-Ku80 was included as a control. Images were obtained before and 3 min after irradiation. Scale bars, 2 μm.

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