Figure 6: PAXX promotes non-cohesive end joining. | Nature Communications

Figure 6: PAXX promotes non-cohesive end joining.

From: Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway

Figure 6

(a,b) PAXX stimulates non-cohesive end ligation in the presence of XLF. The linear DNA substrates contain blunt–3′ overhangs (EcoRV–KpnI) (a) or blunt–blunt (EcoRV–EcoRV) (b) ends. The reactions contain 5 nM Ku70/80, 2.5 nM XRCC4–Lig4 and the indicated wild-type PAXX or its mutant F201A, with or without 2.5 nM XLF. Data from three independent experiments are represented as the mean±s.e.m.

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