Figure 7: A model showing the relationship of PAXX and XLF in DSB repair. | Nature Communications

Figure 7: A model showing the relationship of PAXX and XLF in DSB repair.

From: Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway

Figure 7

(a) IR induces DSBs with complex ends containing multiple modifications (‘dirty ends’). Cells require a full NHEJ complex, including both PAXX and XLF, to stabilize the alignment of ends for a ‘trial and error’ process with multiple modification enzymes. (b) A Topo2 inhibitor, VP16 or doxorubicin, will induce the formation of a Topo2–DNA cross-linked complex. The Topo2 peptide will be removed by TDP2 to generate a cohesive end that is phosphorylated at its 5′-end. These ‘clean’ ends can be quickly ligated even by an incomplete NHEJ complex lacking XLF or PAXX.

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