Figure 1: Loss of Roquin causes T cell-autonomous miRNA accumulation.
From: Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis
(a) Flow cytometric stains from Roquinsan/san and Roquin+/+ T cells showing the gating strategy used to sort CD4+CD44loCD25− naive T cells. (b) Scatter plot of microRNA expression in sorted naive T cells obtained by microarray analysis. (c) qRT–PCR showing relative expression levels of mature miR-146a and miR-21 in naive T cells, normalized to U6. (d) Relative expression levels of miR-146a measured by qRT–PCR in Rc3h1fl/fl;Rc3h2fl/fl; CD4-Cre double knockout naive T cells normalized to snoRNA202. (e,f) qRT–PCR analysis of miR-146a (e) and miR-21 (f) in Roquinsan/san.Ly5b: Roquin+/+.Ly5a or of Roquin+/+.Ly5b: Roquin+/+.Ly5a mixed chimeras. Each dot represents an individual mouse and the bar represents the median value in each group. U-test: *P<0.02, **P<0.05, ***P<0.005. See also Supplementary Fig. 1a,b.
