Figure 3: Roquin enhances Dicer mediated pre-miR-146a processing.

(a) In vitro processing of pre-miR-146a in HEK293T cells after transfection with FLAG-Dicer (lane 1), Roquin^wt-GFP (lane 3) or Roquin^san-GFP (lane 4). Co-transfection of FLAG-Dicer with either Roquin^wt-GFP (lane 5) or Roquin^san-GFP (lane 6) is included. No transfection control is shown in lane 2. (b) In vitro processing of pre-miR-146a in HEK293T cells after transfection with FLAG-Dicer (lane 2) alone, or co-transfection of FLAG-Dicer with either Roquin^wt-GFP (lane 3) or Roquin^san-GFP (lane 4). Lane 1: No transfection control. The lower panels show densitometric analysis of the northern blots to quantify ³²P. The ratios of the mature versus precursor miRNA intensities are plotted. Intermediates or low abundance compared with the fully digested products were not included in the quantification.