Figure 4: Roquin regulates miR-146a stability in vitro and in vivo.

(a) miR-146a in Roquin^san/san and Roquin^+/+ naive T cells measured by qRT–PCR and normalized to comparatively stable U6 snRNA. The cells were treated with actinomycin D (10 mg ml⁻¹) for the times indicated. The amount of miR-146a at 0 h was assigned 100%. The error bar represents the range of the data for two experiments. (b) Pre-miR-146a was transfected along with Roquin^wt-GFP or Roquin^san-GFP in HEK293T cells and qRT–PCR for miR-146a was performed on GFP-positive cells on days 2 and 5 post transfection. See also Supplementary Fig. 2a. (c) miR-146a in MEFs 7 days after retroviral transduction of precursor miR-146a along with Roquin^wt or Roquin^san. Each dot represents a technical replicate and the bars represent the median value in each group. U-test: *P<0.01, **P<0.001, NS, not significant. The data are representative of two independent experiments.