Figure 4: P and NO3− provisions influence HRS1 protein accumulation, but the P signal does not affect its mRNA accumulation and NO3− response. | Nature Communications

Figure 4: P and NO3− provisions influence HRS1 protein accumulation, but the P signal does not affect its mRNA accumulation and NO3− response.

From: AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis root tip

Figure 4

(a) HRS1–GFP protein accumulation is induced in response to 1 mM NO3− treatment. (b) HRS1–GFP protein accumulation is affected by P provision; 48 h P starvation (reported by IPS1 sentinel regulation) does not affect HRS1 (endogenous gene), HHO1, and HRS1:GFP mRNA accumulation. (c) Rapid NO3− transcriptional activation of HRS1 and subsequent protein accumulation is maintained regardless P provision. Different NO3− levels induce: (i) HRS1–GFP protein accumulation in roots of 48-h P starved plants and (ii) HRS1 transcript accumulation in plant roots grown (14 days) on P varying media. Values are means±s.e.m. (n=3).

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