Figure 1: Dynamic cell cycle progression of virus-specific CD8+ T cells shown by Fucci probes.
From: Real-time tracking of cell cycle progression during CD8+ effector and memory T-cell differentiation
(a) Fucci fluorescent signals in cell cycle dynamics. The originally designed Fucci system, in which mutually expressed Fucci probes correspond to each cell cycle phase. The cells in G0/1 show high intensity of mKO2-hCdt1(30/120), and S/G2/M cells represent the accumulation of mAG-hGem(1/110). (b) Magnetic-activated cell sorting-purified Fucci/OT-I (CD45.2+) cells were labelled with Cell Trace Violet (CTV) dye and transferred into CD45.1+ recipients prior to intranasal (i.n.) PR8-OVA influenza A virus infection. Representative flow plots show the CTV dye dilution profile and mAG versus mKO2 expression level. Bottom panels depict the percentage of mKO2+ (orange lines) or mAG+ (blue lines) from all mice (n=9). Data are presented as means±s.e.m.
