Figure 3: Proliferating virus-specific CD8+CD62Lint cells segregate into CD62Lhi and CD62Llo subsets.
From: Real-time tracking of cell cycle progression during CD8+ effector and memory T-cell differentiation
Magnetic-activated cell sorting-purified Fucci/OT-I (CD45.2+) cells were labelled with CTV dye and transferred to CD45.1+ recipients prior to PR8-OVA influenza A virus infection. (a) Surface expression of CD62L against CTV dilution on virus-specific CD8+ T cells in MLNs and spleens on days 2, 3, 4 and 7 p.i. Data are representative of three independent experiments with three to five mice each. (b) CD62Lint Fucci/OT-I cells in the 4th–8th divisions were sorted from splenocytes on day 4 p.i. and transferred into the concurrently infected 2nd recipients. Ten days after transfer, 2nd recipients were analysed for the change of CD62L level. The percentage of CD62L mean fluorescence intensities against naive CD8+ T cells is shown to compensate differences in fluorescence between sorter and analyzer instruments. Symbols show mean with s.d. (n=7). Data are representative of three independent experiments with three mice each.
