Figure 5: In-vivo validation of dsDMR enhancers.
From: Developmental enhancers revealed by extensive DNA methylome maps of zebrafish early embryos
(a) Epigenome Browser69,70 view of the six3a gene, neighbouring dsDMR enhancers (six3a-e1 and six3a-e2, grey boxes) and cloned region for a negative control (six3a-ne1 and six3a-ne2, dark grey boxes). (b) GFP expression driven by the dsDMR enhancers of the six3a gene. Scale bar, 200 μm. (c) The methylation profiles of the six3a promoter and neighbouring dsDMR enhancers (blue and red lines, left y axis) and the expression profile of the six3a gene (green line, right y axis). (d) Epigenome Browser view of the fgfr2 gene, neighbouring dsDMR enhancers (fgfr2-e1, fgfr2-e2, fgfr2-e3 and fgfr2-e4, grey boxes) and cloned regions for negative controls (fgfr2-ne1, fgfr2-ne2, fgfr2-ne3 and fgfr2-ne4, dark grey boxes). (e) GFP expression driven by the dsDMR enhancers of the fgfr2 gene. (f) The methylation profiles of the fgfr2 promoter and neighbouring dsDMR enhancers (blue and red lines, left y axis) and the expression profile of the fgfr2 gene (green line, right y axis). *Only one G1 transgenic line was established. Forebrain (f); midbrain–hindbrain boundary (mh).
