Figure 7: H/I-induced brain damage is reduced in LysM-Hif-1α−/− mice.
From: The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia
(a) RT–PCR analysis was performed in primary microglia cultured from HIF-1α+f/+f or LysM-Hif-1α−/− mice using the indicated primers. (b) TIM-3 transcript levels were examined in bran tissues from the contralateral cortex and ischaemic ipsilateral cortex of HIF-1α+f/+f or LysM-Hif-1α−/− mice (n=3) at 24 h post-H/I. (c) Representative images of TTC-stained brain slices from in H/I-induced HIF-1α+f/+f (n=12) or LysM-Hif-1α−/− (n=12) mice 24 h after injury. The infarct volume was quantified with Image J analyser and expressed as a percentage of the damaged ipsilateral hemisphere. (d) Representative confocal microscopic images of immunohistochemical staining for NeuN and cleaved caspase-3 in coronal brain sections from H/I-induced HIF-1α+f/+f or LysM-Hif-1α−/− mice 24 h after injury. Scale bar, 50 μm. The graph shows the mean number of NeuN and cleaved caspase-3-stained cells per mm2 (±s.d. from three independent experiments).
