Figure 3: Regulatory asymmetry between myoepithelial and luminal cell types.
From: Epigenetic and transcriptional determinants of the human breast
(a) Plot of the relative abundance of TF binding sites overlapping UMRs in luminal (lum) and myoepithelial (myo) cells reveals regulatory asymmetry between the breast cell types. Red line represents total UMR abundance. (b) Fraction of DE genes (upregulated in luminal: red, upregulated in myoepithelial: green) associated with luminal (bottom panel) and myoepithelial (top panel) UMRs. Left panel shows proximal UMRs (UMRs within transcriptional start site (TSS) +/− 2 kb), and right panel shows distal UMRs (TSS +/− 20 kb). (c) Fraction of DE genes (upregulated in luminal: red, upregulated in myoepithelial: green) associated with both luminal and myoepithelial UMRs. Left panel shows proximal UMRs (UMRs within TSS +/− 2 kb) and right panel shows distal UMRs (TSS +/− 20 kb). (d) Differential expression (upregulated in luminal: red, upregulated in myoepithelial: green) of proximal UMRs overlapping with binding sites of luminal enriched (FoxA1, Gata3 and Znf217), and myoepithelial enriched TFs (Egr1). The size and shade of the circles represents fold change between luminal and myoepithelial gene expression on the log2 scale. (e) RNA yield (microgram per million cells) from luminal (red) and myoepithelial (green) cells extracted from three individuals.
